Somayeh Shokri; Azadeh Rasooli; Mehdi Hedayati
Volume 6, Issue 4 , June 2018, , Pages 105-112
Abstract
Abstract After iron, iodine deficiency is the most common nutritional deficiency in third world countries. Today, the urinary iodine which indicates the recent iodine status is the only biological indicator for iodine. Therefore, providing of a long-term indicator for assessing of iodine status is essential. ...
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Abstract After iron, iodine deficiency is the most common nutritional deficiency in third world countries. Today, the urinary iodine which indicates the recent iodine status is the only biological indicator for iodine. Therefore, providing of a long-term indicator for assessing of iodine status is essential. In this study, by using the well-known Sandell–Kolthoff reaction the iodine levels were simultaneously measured in hair and urine of 50 samples. The precision, accuracy, and sensitivity of the assay were also evaluated. Acid digestion was sufficiently accurate. Intra and inter-assay coefficients were less than 10 and 12 percent, respectively. Based on the recovery and recycling tests, the recovery percentage of the applied method was 90 to 110 percent which indicates an acceptable accuracy for this study. This method can digest the hair samples in an acidic environment and has enough efficiency to remove interfering and iodine releasing agents in the samples. Moreover, this method has sufficiently the precision, accuracy, and sensitivity required for the measurement of iodine in the hair samples. In addition, using of acidic digestion, reduces the risk of alkaline medium for digestion and the time of long-term ashing methods. It also increases the safety of the used method.
Reza Hajihosseini; Akram Bayati; Azadeh Rasooli; Atoosa Vaziri
Volume 6, Issue 3 , March 2018, , Pages 49-59
Abstract
Background and Objective: In the present study, we investigated the protective effects of Zataria multiflora essential oil against iron nano particle induced liver damage in rats. The animals were divided into three groups: negative control group; animals were treated with normal salin in three days. ...
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Background and Objective: In the present study, we investigated the protective effects of Zataria multiflora essential oil against iron nano particle induced liver damage in rats. The animals were divided into three groups: negative control group; animals were treated with normal salin in three days. poitive control group: 200 mg/kg b.w iron nano particle was administered intrapertoneally to male in three days. Treatment group with Zataria multiflora oil; animals were injected intraperitoneally with Zataria multiflora oils (100 and 200 mg/kg b.w) in three days. After three days, blood samples were drawn from heart and the liver tissues were removed for biochemical and histological studies. The hepatoprotective activity was assessed using various biochemical parameters such as alanine aminotransferase (ALT), aspartate amino transferas (AST), alkalin phosphatase (ALP), Ferric Reducing Ability of Plasma (FRAP), lipid peroxidation (LP) and glutathione (GSH). The results of this study showed that iron nano particles caused increasing the serum AST and LP levels and decreasing FRAP and GSH levels, but it has no effect on ALT and ALP. Consumption of Zataria multiflora oil reversed AST, LP and GSH levels to the normal levels without any effect on FRAP. Finally, treatment of rats with iron nanoparticle caused induction of oxidative hepatic damage and consumption of zataria essential oil can be effective for the recovery and prevent of liver damage.
